The scanned tiff images were analyzed numerically and background corrected using the agilent feature extraction software. Rna was applied microarray agilent technologies, 8. The surescan dx microarray scanner system is indicated for. The file lists the location of each probe in the design from back, left orientation along with its accession number, gene name, performance score, and other information. Agilent technologies 11 surescan microarray scanner system microarray scan control software 9. Output format of agilent feature extraction and agilent genomic workbench agw software. The agilent dna microarray scanner is a 48slide scanning system enabled by surescan highresolution technology capable of scanning with 2, 3, 5 or 10 micron resolution that can read 1 x 3 glass slide microarrays and seamlessly feature extract them all using agilents feature extraction software.
Agilent surescan g2600d microarray scanner g4900da g2600d is part number of scanner itself, and g4900da is a system of surescan g2600d scanner and computer with software. The microarray scanner is a sophisticated laser induced fluorescence scanner designed to read microarrays printed on standard 1 in x 3 in slides. Agilent surescan microarray scanner the new agilent surescan microarray scanner is the foundation of our complete microarray solution and represents the latest innovation in. There is a builtin ozone free environment to prevent the slides from photo bleaching. Agilent s dna microarray scanner with surescan highresolution technology is the key component of microarray based applications in which increased coverage of the genome is a necessity. It includes the surescan microarray scanner, software and accessories, the g2565 series microarray scanner software and accessories. The surescan microarray scanner is a laser induced fluorescence scanner designed to read microarrays printed on a standard 1 inch x 3 inch glass slides. Agilent g2565ba microarray scanner iet refurbished. Agilent s reagent rental program offers a flexible and affordable solution for microarray analysis using the complete agilent platform. In the subsequent data preprocessing, raw intensity data from the spotted arrays and agilent arrays were treated equally. The array slide was scanned using an agilent g2565ca microarray scanner system, and probe signals were quantified using agilent s feature extraction software version 9. Labeled crna was hybridized to a sureprint g3 mouse ge 8.
Apr 12, 2018 after scanning the hybridized slides using the agilent g2565ca microarray scanner and scan control software 8. Agilent 2100 bioanalyzer g2939a with control system and software. Product dna microarray scanner with sure scan highresolution technology, model g2565ca. Microarray scanners are devices used to measure the fluorescent areas of a dna microarray to reveal information about the simultaneous activity of thousands of genes. Hybridized microarray slides were scanned with an agilent dna microarray scanner g2505c and data was analyzed with the agilent scancontrol version 8. G2600d is part number of scanner itself, and g4900da is a system of surescan g2600d scanner and computer with software. Please contact us for complete warranty, service contract and usedrefurbished agilent g2565 ca system details. Refurbished agilent g2565ba microarray scanner pn g2505b complete with 48 position carousel, feature extraction and analysis software, and pc data system. The probe intensities were extracted using the agilent feature extraction software, and. Please contact us for complete warranty, service contract and usedrefurbished agilent g2565 ca. The agilent dna microarray scanner is a 48slide scanning system enabled by surescan highresolution technology capable of scanning with 2, 3, 5 or 10 micron resolution that can read 1 x 3 glass slide microarrays and seamlessly feature extract them all using agilent s feature extraction software with robust statistical analyses. Agilent dna microarray scanner agilents dna microarray scanner with surescan highresolution technology is the key component of microarraybased applications in which increased coverage of the genome is a necess. Preprocessing and differential expression analysis of agilent microrna arrays using the agimicrorna bioconductor library. The surescan microarray scanner is a key component of agilents complete microarray solution.
Vector network analyzer vna software for the control of hp, agilent and keysight vector network analyzer, such as the 8753, 8720. The scanned tiff images were analyzed numerically and background corrected using the agilent feature extraction software version 10. The surescan dx microarray scanner system, consisting of surescan dx microarray scanner with autoloader and agilent microarray scan control software, is intended to measure fluorescence signals of labeled dna and rna target hybridized to microarrays. Fluorescently labeled targets were hybridized to agilent rice oligo microarrays for 17 h at 65c. Raw intensity data were obtained using agilent s feature extraction software version 10. Identification of novel genetic markers for mouse yolk sac. Identification of novel differentially expressed lncrna and. Genomewide profiling to analyze the effects of high fat. Find great deals on a wide selection of microwave digesters, reagents, synthesis, and microarray scanners in auctions, and classified for sale ads on labx. The agilent surescan dx microarray scanner is cemarked for in vitro diagnostic ivd use in many countries outside the us. Comparative analysis of naive, primed and ground state. When buying a microarray scanner, important features to consider are sensitivity, resolution, scan area, rapidity of scan. Using data from 243 breast cancer samples analyzed on two different microarray platforms, our inhouse 29k array platform and agilent sureprint g3 microarray platform, we. Features special to this model are highlighted yellow.
Surescan microarray scanner high sensitivity and resolution. Agilent 2100 bioanalyzer g2938c with control system and software condition. Whether performing gene expression studies, array cgh, mirna pro. Quantile normalization and differential analysis were performed with the agilent genespring software v. Evaluation of highresolution scanners for analyzing. The microarrays were scanned using an agilent g2565ca microarray scanner with the scan control software v8.
The microarray scanner is a sophisticated laser induced fluorescence scanner designed to read microarrays. Jul 03, 2014 the scanned images of the spotted arrays were quantified by gene pix pro 6. Scan control software is the user interface software that controls the agilent scanner. Agilent g2565ca microarray scanner system spectralab. If you will be using a 3rd party microarray scanner other than the axon genepix, the scanner s software will need this file to process the images. Agilent s feature extraction software reads and processes up to 100 raw microarray image files in an automated, walkaway mode. Surescan microarray scannerg4900da from agilent technologies description agilents surescan microarray scanner enables automated highspeed scanning and is able to produce highquality data from both legacy and upcoming arrays. Notice that the feature is clearly distinguished on the agilent microarray scanner, but is barely distinguishable on the brand y scan, and is not detectable at all on the brand z scan. After hybridization, following wash processes were performed according to the manufacturers instructions, and hybridized microarrays were scanned using an agilent microarray scanner g2565ca. Find agilent, tecan, and invitrogen for sale on machinio. With improved feature resolution, it delivers exceptional flexibility to analyze any microarray. System description in this section learn about the hardware and software features of the microarray scanner, the parts and system requirements. The image on a chip was acquired with a g4900da surescan microarray scanner agilent technologies, ca, usa and analyzed with agilent cytogenomics software agilent technologies for chromosome gain or loss.
Microarray scanners microarray scanner systems biocompare. By agreeing to purchase a predefined volume of microarrays and reagents over the course of a specified agreement period, agilent will loan your lab a complete system including the scanner, hybridization oven, hybridization chambers, and software. Surescan microarray scannerg4900da from agilent technologies. The microarray scanner is a sophisticated laserinduced fluorescence scanner designed to read microarrays printed on standard 1 in. Agilent technologies agilent g4900da surescan microarray scanner system microarray scan control software 9. File format used for the storage and exchange of microarray data and metainformation. Functional role of a novel long noncoding rna in esophageal. G2565ca feature description dynamic range 104 16bit data format, 105 20bit data format, 106 with xdr scanning dynamic autofocus continually adjusts scanners focus, keeping features in focus at all times autoloa. Surescan dx microarray scanner by agilent technologies, inc. The agilent dna microarray scanner is a 48slide scanning system enabled by surescan highresolution technology capable of scanning with 2, 3, 5 or 10 micron resolution that can read 1 x 3 glass slide microarrays and seamlessly feature extract them all using agilent s feature extraction software. Aberrations were detected by using default setting. The probe intensities were extracted using the agilent feature extraction software, and probe intensities were background corrected and normalized quantile normalization using the limma package in r.
The signal level of the feature shown is 8 counts and 58 counts over background in the red and green channels, respectively, on the agilent microarray scanner. Here is an agilent surescan g2600d microarray scanner g4900da. We carry used and preowned dna sequencing systems and agilent bioanalyzers. The agilent surescan scanner provides a precise and efficient microarray scanner system for many research applications. Tecans powerscanner is the microarray scanner of choice for highdensity dna microarrays and protein microarrays.
The array slide was scanned using an agilent g2565ca microarray scanner system, and probe signals were quantified using agilents feature extraction software version 9. Microarray scanners microarray scanner systems microarray scanners are widely used in functional biology, cellomics, gene expression and drug discovery research. G2565ca feature description dynamic range 104 16bit data format, 105 20bit data format, 106 with xdr scanning dynamic autofocus continually adjusts scanner. The microarray scanner measures the fluorescence intensity of. Data were extracted on a g2565ca dna microarray scanner using the agilent feature extraction software agilent technologies with default settings.
Agilent technologies microarray scanner approved for in vitro diagnostics in china. We ship our instruments immediately, delivery can take up to 4. Agilent oligonucleotide arraybased cgh for single cell. Dna microarray scanner dna microarray scanner g2565ca. Agilent s widely used cgh array, followed by data analysis using a novel algorithm implemented within agilent s genomic workbench software. The software automatically finds and places microarray grids, rejects outlier pixels, accurately determines feature intensities and ratios, flags outlier pixels, and calculates statistical confidences. Agilent s surescan microarray scanner enables automated highspeed scanning and is able to produce highquality data from both legacy and upcoming arrays. Agilent g2565ca microarray scanner system scan control software.
This software is usable only with the agilent highresolution scanner g2565ca or highresolution scanner upgrade. Data analysis cytogenomics software g1662aag1667aa agilent microarrays, reagents and scanner are. Agilent microarray scanner and feature extraction software. Gse75471 osmyc2responsive genes in osmyc2overexpressing. Surescan microarray scanner for flexible analysis for genomics and cytogenetics microarrays. Data were extracted on a g2565ca dna microarray scanner using the agilent feature extraction software agilent. Product dna microarray scanner with sure scan highresolution technology, model g2565ca special features. The highresolution scanner released by agilent is the model with id number g2565ca which can scan highdensity microarrays up to 2 um resolution figure 1. Dna microarray scanner agilent g25265ca with sure scan high resolution technology. Agilent g5761a surescan dx microarray scanner system. Agilent microarray scan control icon when the program starts, the agilent microarray scan control program main window opens and the scanner performs its initialization sequence. This section describes the features that make the microarray scanner a powerful tool for agilents microarray analysis solution. Agilent g2565ca microarray scanner system for sale. Guida alluso dello scanner per microarray agilent g2565ca software scan control 8.
After hybridization and washing, the slides were scanned using an agilent g2565ca microarray scanner. The image on a chip was acquired with a g4900da surescan microarray scanner agilent technologies, ca, usa and analyzed with agilent cytogenomics software agilent technologies for. Both the limma and the agilp packages use the output for the red and green channels from the agilent feature exrtaction software as inputs, as far as i can tell, and i dont have them. Jan 10, 20 fluorescently labeled targets were hybridized to agilent rice oligo microarrays for 17 h at 65c. Contains three subtables, storing the feature extraction parameters, microarray level statistics, and featurelevel data. Agilent surescan g2600d microarray fluorescence scanner. The scan control program communicates with the scanner. Pdf preprocessing and differential expression analysis of. The agilent dna microarray scanner is a 48slide scanning system enabled by surescan highresolution technology capable of scanning with 2, 3, 5 or 10 micron resolution that can read 1 x 3 glass slide microarrays and seamlessly feature extract them all using agilents feature extraction software with robust statistical analyses.
Data analysis cytogenomics software g1662aag1667aa agilent microarrays, reagents and scanner. Designed for research using microarrays, the scanner system provides a laserinduced fluorescence scanning solution designed to read microarrays printed on standard 1 inch by 3 inch glass slides. The scanner is equipped with new optics, electronics, and software. Microarray scanners highquality array scanning systems.
Agilent dna microarray scanner g2565ca table contains all key specifications for the dna microarray scanner, model ba. Agilent g2565ca dna microarray scanner iet refurbished. Used agilent surescan g2600d microarray scanner g4900da. Current listings 4396 labx member since 6th october, 2004 15 years ago seller location markham, ontario, canada shipping policy. The surescan dx microarray scanner is class ii, 510k exempt in the us. Data analysis software 2 perpetual licenses of agilent feature extraction included approximate scanner. The agilent g2565ca microarray scanner is part of the microarray analysis solution from agilent technologies. I have been planing to use the bioconductor package to perform the analysis, but i am at a loss as to how to go about doing this. Refurbished agilent g2565ca dna microarray scanner pn g2505c with surescan highresolution technology. A crossplatform comparison of affymetrix and agilent. Arrays were scanned with an agilent g2565ca microarray scanner system agilent, santa clara, ca.
It measures the fluorescence intensity of two dyes simultaneously from labeled sample nucleic acid bound to microarrays. Agilent g2565ca microarray scanner system scan control software 8. Microarray design files available for download agilent. Identification of novel differentially expressed lncrna. G2565 series microarray scanner software and accessories. An economical tool for genetic screening of large global populations. Its ability to correctly identify and focus on the arrayed surface of glass slides, including nitrocellulosecoated and reflective surfaces, makes it a uniquely versatile microarray scanner.
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